Soon after incubation at four °C for 30 min, the purified protein was concentrated by Amicon® Ultra Centrifugal Filters . Then, the protein was diluted 10-fold with EDTA-free 50 mM MES buffer (pH six.5) and applied to Ultra filters once again to be concentrated to its original volume. The process was repeated for 5 occasions to take away EDTA in the protein buffer. SH-SY5Y cells were used to test the impact of metals on mammalian TH phosphorylation at Ser40. Antibodies…
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